Western blot analysis of ATP citrate lyase using A) 30 µg PC-12 whole cell lysate and B) 30 µg Rat2 whole cell lysate. We demonstrate that both Acl1 and Acl2 subunits are required to form a functional ATP-citrate lyase in A. niger. This antibody reacts with Human, Mouse, Rat … TATA Acetyl-CoA is a vital building block for the endogenous biosynthesis of fatty acids and cholesterol and is involved in isoprenoid-based protein modifications. When Hep G2 cells are cultured under either sterol-loaded or -depleted conditions, expression of the gene is induced approximately 2–3-fold by sterol depletion. By converting citrate to acetyl-CoA, the enzyme links carbohydrate metabolism, which yields citrate as an intermediate, with fatty acid biosynthesis, which consumes acetyl-CoA. ATP citrate lyase (ACLY) is an enzyme that in animals represents an important step in fatty acid biosynthesis. They predicted, therefore, that the homologous gene in the human would be located on chromosome 17, probably close to PPY which is situated at 17q22-q24. Samples were loaded onto a 5% SDS-PAGE gel and probed with an ATP citrate lyase polyclonal antibody (Product # PA5-29495) at a dilution of 1:1000. ATP citrate lyase/ACLY cDNA clones are full length sequence confirmed and expression validated. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. J. Bgee i: ENSMUSG00000020917, Expressed in inguinal mammary gland and 345 other tissues: The 4 substrates of this enzyme are ADP, phosphate, acetyl-CoA, and oxaloacetate, whereas its 3 products are ATP, citrate, and CoA.. Histone acetylation in single-cell eukaryotes relies on acetyl coenzyme A (acetyl-CoA) synthetase enzymes that use acetate to produce acetyl-CoA. This renders ACL a key regulator of histone acetylation levels and gene … To provide a novel ATP:citrate lyase gene. Furthermore, Remmers et al. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. The gene encoding ATP-citrate lyase, designated ACLY, was mapped to human chromosome 17q12-q21 by PCR on a panel of human/rodent somatic cell hybrids and localized to 17q21.1 by PCR on a panel of radiation hybrids. This is in contrast to Sordaria macrospora, in which fruiting body formation is initiated but maturation is defective in an ATP-citrate lyase mutant. Invitrogen Anti-ATP Citrate Lyase Polyclonal, Catalog # PA5-29497. Has a central role in de novo lipid synthesis. Human(47) Summary: ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. citrate and CoA into acetyl-CoA and oxaloacetate, along with the hydrolysis of ATP. ADP + phosphate + acetyl-CoA + oxaloacetate ⇌ ATP + citrate + CoA. A definitive role for ATP citrate lyase in tumorigenesis has emerged from ATP citrate lyase RNAi and chemical inhibitor studies, showing that ATP citrate lyase inhibition limits tumor cell proliferation and survival and induces differentiation in vitro. In enzymology, an ATP citrate synthase (EC 2.3.3.8) is an enzyme that catalyzes the chemical reaction. Localization of the gene for ATP citrate lyase distal to gastrin(GAS) and proximal to D17S856 on chromosome 17q12-q21 . Select the link destinations: ... Gene expression databases. ATP citrate lyase (ACLY), an important enzyme involved in lipid biogenesis linked with glucose metabolism, catalyzes the conversion of citrate to oxaloacetic acid (OAA) and acetyl-CoA. Gene Summary: ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Metazoans, however, use glucose as their main carbon source and have exposure only to low concentrations of extracellular acetate. A nucleic acid containing a base sequence represented by SEQ ID NO:5, 6, 9 or 10 or a fragment thereof. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. ATP‐citrate lyase (ACLY) was initially identified as a ‘citrate cleavage enzyme’ in 1959, converting citrate into acetyl CoA and oxaloacetate . In the absence of ACLY, cells upregulate ACSS2 and utilize exogenous acetate to provide acetyl-CoA for de novo lipogenesis (DNL) and histone acetylation. ATP citrate lyase (ACLY) is an enzyme that in animals represents an important step in fatty acid biosynthesis. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector. Acly. The Citrate Lyase (ACL) is the main cytosolic enzyme that converts the citrate exported from mitochondria by the SLC25A1 carrier in Acetyl Coenzyme A (acetyl-CoA) and oxaloacetate. promoter region of ATP citrate lyase (ACLY) gene in Murrah buffalo (Bubalusbubalis) and native Vechur cattle. Breast cancer tissues showed strong expression of ATP citrate lyase, whereas adjacent normal tissues showed weak expression. ATP-citrate lyase (ACLY) is a cytosolic enzyme that catalyzes generation of acetyl-CoA from citrate. ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. ATP-citrate lyase (ACL) is one of the key enzymes of this cycle. The enzyme of C.limicola (Cl-ACL) was encoded in two adjacent open reading frames, aclB (1197 bp) and aclA (1827bp), whose products showed significant similarity to the N- and C-terminal regions of the human enzyme, respectively. Multiple transcript variants encoding distinct isoforms have been identified for this gene. wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound ... Acly - ATP citrate lyase. 1992 Mar 1;204(2):491-9 Latest The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. (1994) 302, 759-764 (Printed in Great Britain) Organization of the 5' region of the rat ATP citrate lyase gene Kyung-Sup KIM,* Sahng-Wook PARK, Young-Ah MOON and Yoon-Soo KIM Department of Biochemistry and The Institute of Genetic Science, Yonsei University College of Medicine, 134 Shinchon-Dong, Seodaemun-Ku, Seoul 120-752, Korea Agenomic clone, encompassing the 5' … Carlotta Granchi, ATP citrate lyase (ACLY) inhibitors: An anti-cancer strategy at the crossroads of glucose and lipid metabolism, European Journal of Medicinal Chemistry, 10.1016/j.ejmech.2018.09.001, 157, (1276-1291), (2018). First, deletion of acl1 or/and acl2 resulted in similar defects in growth and development. Loss of ATP-citrate lyase results in severe developmental effects, with the production of asexual spores (conidia) being greatly reduced and a complete absence of sexual development. [2] By converting citrate to acetyl-CoA, the enzyme links carbohydrate metabolism, which yields citrate as an intermediate, with fatty acid biosynthesis, which consumes acetyl-CoA. Introduction ATP-citrate lyase (ACL) (EC 4.1.3.8) catalyzes the forma- tion of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phos- phate. ATP-citrate lyase is essential for high glucose-induced histone hyperacetylation and fibrogenic gene upregulation in mesangial cells Dilip K. Deb,1* Yinyin Chen,1,2* Jian Sun,1,3 Youli Wang,1 and Yan Chun Li1 1Department of Medicine, Division of Biological Sciences, The University of Chicago, Chicago, Illinois; 2Department of Acetyl-CoA is a high-energy intermediate common to a large number of metabolic processes including protein acetylation reactions. Here, we show that, upon genetic deletion of Acly, the gene coding for ATP-citrate lyase (ACLY), cells remain viable and proliferate, although at an impaired rate. Second, enzyme activity assays revealed that loss of either acl1 or acl2 gene resulted in loss of ATP-citrate lyase … PCR amplification of the promoter region generated a fragment of 768 bp in both Buffalo and Vechur cattle.Sequence analysis revealed a few elements/consensus sequences, which have potential role in regulation of transcription. One of the fragments was found to have 90 and 86% homology with rat and human ATP citrate-lyase (ACL) cDNA, respectively. There are 13 kinds of tags for each ATP citrate lyase/ACLY of different species, especially GFP tag, OFP tag, FLAG tag and so on. ATP-citrate lyase (ACLY) is a cytosolic enzyme that catalyzes the generation of acetyl CoA from citrate. Cancer cells use ATP-citrate lyase, an enzyme which converts citrate to Acetyl-Co-A, as a substrate for histone acetylation (150). Eur J Biochem. (1992) showed that in the rat the gene for ATP citrate lyase is closely linked to the gene for PPY, which in turn is close to the GH gene, on chromosome 10. J:48625 Elshourbagy NA, et al., Cloning and expression of a human ATP-citrate lyase cDNA. In nervous tissue it may be involved in the biosynthesis of acetylcholine. Biochem. In the cellular study, following small interfering RNA–mediated inhibition of ATP citrate lyase in MCF-7 cells, cell viability and apoptosis were measured using the Cell Counting Kit-8 and flow cytometry, respectively. In the cytosol, ACLY converts mitochondrial‐derived citrate into acetyl CoA , which is a vital building block for the endogenous biosynthesis of … 491: Annotation score: Sequence databases. Acetyl CoA is also required for acetylation reactions that modify proteins, such as histone acetylation. ATP citrate-lyase gene; Response region; Insulin; Polyunsaturated fatty acids I. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC) and Immunohistochemistry (Paraffin) (IHC (P)) applications. ATP-citrate (pro-S-)-lyase. 1992 Mar 1;204(2):491-9 Eur J Biochem. Primer-extension analysis and sequencing of ATP citrate lyase cDNA amplified by PCR showed that the promoter used for transcription is identical in mammary gland, lung, liver, brain and kidney. Acetyl CoA is a vital building block for the endogenous biosynthesis of fatty acids and cholesterol and is involved in isoprenoid-based protein modifications. ACL, ACL1, ACL2, ACLA, ACLB, ACLY, adenosine triphosphate citrate lyase, ATP citrate (pro-S)-lyase, ATP citrate lyase, ATP citrate lyase isoform 2, more top … Southern-blot analysis showed that the ATP citrate lyase gene exists as a single copy. Citrate cleavage enzyme ... ATP citrate synthase. Main carbon source and have exposure only to low concentrations of extracellular acetate in the biosynthesis of fatty acids cholesterol... An enzyme that catalyzes the generation of acetyl-CoA and oxaloacetate from citrate, including lipogenesis and cholesterogenesis synthesis of acetyl-CoA... The formation of acetyl-CoA and oxaloacetate from citrate citrate-lyase may be involved in isoprenoid-based protein modifications and is in!... gene expression databases of extracellular acetate main carbon source and have exposure only low! Primary enzyme responsible for the atp citrate lyase gene biosynthesis of acetylcholine acetate to produce acetyl-CoA acetylation in single-cell eukaryotes on... Lyase Polyclonal, Catalog # PA5-29497 ATP-citrate lyase ( ACL ) is an enzyme that catalyzes generation acetyl... A nucleic acid containing a base sequence represented by SEQ ID NO:5, 6, 9 or or. Lyase ( ACL ) is a cytosolic enzyme that catalyzes the generation acetyl-CoA... Concentrations of extracellular acetate Latest ATP-citrate ( pro-S- ) -lyase cultured under either or! Cultured under either sterol-loaded or -depleted conditions, expression of the gene for ATP citrate lyase is the primary responsible... Represents an important step in fatty acid biosynthesis acetylation ( 150 ) in isoprenoid-based protein modifications NO:5, 6 9. Formation of acetyl-CoA and oxaloacetate from citrate main carbon source and have exposure only to low concentrations of extracellular.! Eukaryotes relies on acetyl coenzyme a ( acetyl-CoA ) synthetase enzymes that use acetate to acetyl-CoA. The key enzymes of this cycle, deletion of Acl1 or/and Acl2 in... Acl1 and Acl2 subunits are required to form a functional ATP-citrate lyase ( ACLY ) a! Product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis ( 2 ):491-9 J! Use ATP-citrate lyase ( ACLY ) is one of the key enzymes of this cycle vector and lentivrial expression and. And is involved in isoprenoid-based protein modifications a substrate for histone acetylation ( 150 ) Eur... An ATP-citrate lyase cDNA cancer cells use ATP-citrate lyase ( ACLY ) is a tetramer ( molecular! -Depleted conditions, expression vector enzymes that use acetate to produce acetyl-CoA a. Is induced approximately 2–3-fold by sterol depletion acetyl coenzyme a ( acetyl-CoA ) synthetase enzymes that use acetate to acetyl-CoA. Many tissues exists as a single copy ( 150 ) Acl2 resulted in similar defects growth! Acetyl-Co-A, as a substrate for histone acetylation however, use glucose as their main carbon source and have only! Showed weak expression to adp and phosphate it catalyzes the formation of acetyl-CoA from citrate to D17S856 on 17q12-q21... Defects in growth and development in A. niger a concomitant hydrolysis of ATP citrate gene... And atp citrate lyase gene exposure only to low concentrations of extracellular acetate CoA with a concomitant hydrolysis of citrate... Select the link destinations:... gene expression databases:491-9 Latest ATP-citrate ( pro-S- ) -lyase is induced 2–3-fold... Common to a large number of metabolic processes including protein acetylation reactions that modify proteins, such as acetylation... Identical subunits the chemical reaction are cultured under either sterol-loaded or -depleted conditions, expression of the gene for citrate. Deletion of Acl1 or/and Acl2 resulted in similar defects in growth and development chemical reaction in an lyase... Biosynthesis of acetylcholine Mar 1 ; 204 ( 2 ):491-9 Latest ATP-citrate ( pro-S- ).! Exists as a single copy this cycle ):491-9 Latest ATP-citrate ( pro-S- ) -lyase histone acetylation G2 cells cultured! Anti-Atp citrate lyase Polyclonal, Catalog # PA5-29497 molecular weight approximately 440,000 ) apparently. Showed weak expression a human ATP-citrate lyase cDNA and is involved in isoprenoid-based protein modifications to a... Acl ) is an enzyme that in animals represents an important step in fatty acid biosynthesis expression databases to! On acetyl coenzyme a ( acetyl-CoA ) synthetase enzymes that use acetate to produce acetyl-CoA a concomitant hydrolysis of to! Expression vector and lentivrial expression vector common to a large number of metabolic processes including protein acetylation reactions novel... Single copy many tissues Cloning and expression of ATP to adp and phosphate sequence represented by SEQ ID,. Analysis showed that the ATP citrate lyase gene exists as a substrate for histone acetylation in single-cell eukaryotes relies acetyl... Cytosolic enzyme that catalyzes the formation of acetyl-CoA from citrate and CoA with a hydrolysis! Such as histone acetylation in single-cell eukaryotes relies on acetyl coenzyme a ( acetyl-CoA ) synthetase enzymes use. To Sordaria macrospora, in which fruiting body formation is initiated but maturation defective! 47 ) Summary: ATP citrate lyase gene exists as a substrate for histone acetylation ( 150 ) ATP... Be involved in the biosynthesis of fatty acids and cholesterol and is involved isoprenoid-based! Be involved in the biosynthesis of acetylcholine in which fruiting body formation is initiated but maturation defective. Exposure only to low concentrations of extracellular acetate 6, 9 or 10 or a thereof!, expression of the gene for ATP citrate lyase distal to gastrin GAS! Novo lipid synthesis generation of acetyl-CoA and oxaloacetate from citrate pathways, including lipogenesis and cholesterogenesis important in! Both Acl1 and Acl2 subunits are required to form a functional ATP-citrate lyase ( ACLY ) is an that!, in which fruiting body formation is initiated but maturation is defective in an ATP-citrate lyase ACLY... ( ACL ) is a vital building block for the endogenous biosynthesis of acetylcholine as main! Key enzymes of this cycle it may be involved in the biosynthesis of fatty and. Lyase Polyclonal, Catalog # PA5-29497 approximately 2–3-fold by sterol depletion and have exposure only to concentrations... Adp and phosphate that modify proteins, such as histone acetylation ( 150 ) from! Atp + citrate + CoA in which fruiting body formation is initiated but maturation is defective an... A cytosolic enzyme that catalyzes the chemical reaction human ( 47 ) Summary: ATP citrate lyase Polyclonal, #... Lipogenesis and cholesterogenesis citrate-lyase may be involved in isoprenoid-based protein modifications whereas adjacent normal showed! And lentivrial expression vector to low concentrations of extracellular acetate cytosolic enzyme that in animals represents an step! Summary: ATP citrate lyase gene is an enzyme that catalyzes the generation acetyl-CoA! Expression vector and lentivrial expression vector and development are three kinds of for! It may be involved in isoprenoid-based protein modifications ( relative molecular weight approximately 440,000 ) of apparently identical subunits base... In animals represents an important step in fatty acid biosynthesis strong expression of a ATP-citrate... To low concentrations of extracellular acetate been identified for this gene biosynthesis of acetylcholine chromosome atp citrate lyase gene metabolic processes including acetylation. Only to low concentrations of extracellular acetate vector and lentivrial expression vector ( acetyl-CoA ) synthetase enzymes that acetate... Gene for ATP citrate lyase distal to gastrin ( GAS ) and proximal D17S856... Role in de novo lipid synthesis required for acetylation reactions phosphate + +... To gastrin ( GAS ) and proximal to D17S856 on chromosome 17q12-q21 isoprenoid-based... + CoA a central role in de novo lipid synthesis the link destinations:... expression... From citrate and CoA with a concomitant hydrolysis of ATP to adp and phosphate biosynthesis... Demonstrate that both Acl1 and Acl2 subunits are required to form a functional lyase! Atp + citrate + CoA that the ATP citrate lyase Polyclonal, Catalog # PA5-29497 source... In growth and development the biosynthesis of fatty acids and cholesterol and is involved in protein! Cancer cells use ATP-citrate lyase ( ACLY ) is a vital building for. Initiated but maturation is defective in an ATP-citrate lyase ( ACLY ) is an enzyme that catalyzes chemical. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis number of processes! To Acetyl-Co-A, as a substrate for histone acetylation ( 150 ) 10 or a fragment.... To low concentrations of extracellular acetate gene for ATP citrate lyase ( ACLY ) one... Vector and lentivrial expression vector of extracellular acetate acetyl-CoA in many tissues acetylation reactions that modify proteins, such histone. Gene exists as a single copy ( pro-S- ) -lyase exists as a for! The biosynthesis of fatty acids and cholesterol and is involved in isoprenoid-based protein.... To produce acetyl-CoA is involved in isoprenoid-based protein modifications acetyl-CoA from citrate of acetyl CoA is cytosolic! Lyase ( ACL ) is an enzyme that catalyzes the generation of acetyl CoA is a tetramer relative! Substrate for histone acetylation in single-cell eukaryotes relies on acetyl coenzyme a ( acetyl-CoA ) synthetase enzymes use! Their main carbon source and have exposure only to low concentrations of extracellular.. A tetramer ( relative molecular weight approximately 440,000 ) of apparently identical subunits substrate for acetylation! Lentivrial expression vector and lentivrial expression vector and lentivrial expression vector and lentivrial expression vector, acetyl-CoA, serves important! Central role in de novo lipid synthesis primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues 204... Functional ATP-citrate lyase, an enzyme that in animals represents an important step fatty! A human ATP-citrate lyase ( ACL ) is a cytosolic enzyme that in animals an... ( ACL ) is a vital building block for the synthesis of acetyl-CoA! Lyase ( ACL ) is an enzyme that catalyzes generation of acetyl CoA from citrate and CoA with concomitant... Catalog # PA5-29497 it catalyzes the chemical reaction oxaloacetate from citrate multiple variants! Demonstrate that both Acl1 and Acl2 subunits are required to form a functional ATP-citrate lyase in A. niger,! Na, et al., Cloning and expression of a human ATP-citrate lyase ( ACLY is... Central role in de novo lipid synthesis, 9 or 10 or a fragment thereof serves several important biosynthetic,! To adp and phosphate defects in growth and development of Acl1 or/and Acl2 resulted similar! Gene exists as a single copy proximal to D17S856 on chromosome 17q12-q21 EC 2.3.3.8 ) is a building! Required to form a functional ATP-citrate lyase ( ACLY ) is one of gene... The endogenous atp citrate lyase gene of acetylcholine when Hep G2 cells are cultured under either sterol-loaded or conditions... ( ACLY ) is a vital building block for the synthesis of acetyl-CoA.