I water it once a month or so from June to September and don’t fertilize it at all now that the plants are fully established. Antioxidant properties of Sideritis may prevent from Cancer. The mammalian genome encodes 4 FOXO proteins, while genomes of Caenorhabditis elegans and Drosophila melanogaster encode only 1 FOXO homolog, DAF-16 and dFOXO, respectively.7 FOXO is responsible for activating genes involved in various cellular stress responses including autophagy, apoptosis, and stress resistance.8 Therefore, it has been pointed out that increasing cellular expression of these proteins may contribute to prolonging the life span of living organisms. The relative fluorescence intensity was determined using Image J software. 29 as of April 7, 2000 (100) Various medicinal properties have been ascribed to natural herbs. Woody perennial growing to 18 inches, native to the mountains surrounding the Mediterranean sea, especially the Peloponnese. These plants will have a lot more details displayed including an image. In the spring or anytime in warm soils, scarify seed on medium grit sandpaper, then sow in pots or flats. The data are expressed as mean ± standard error. Description This tough, drought and heat-tolerant plant is native to the mountains surrounding the Mediterranean sea, especially the Peloponnese. Methods: Optimization was based on various parameters affecting extraction procedure, such as the duration of microwave radiation, the ratio of plant material mass to the solvent, the type and the concentration of the solvent. L4 larvae of age-synchronized N2 worms were transferred via a platinum wire to mNGM (peptone free) agar plates containing 1 mL of SHWE solution to the final concentrations of 5.0 and 50 µg/mL. The relative fluorescence intensity was determined using Image J software. Create a link to share a read only version of this article with your colleagues and friends. (Lamiaceae) from Macedonia and Albania. From ancient times Sideritis plants have been used in folk medicine, most often in the form of aromatic herbal tea [2–4]. It has been reported that plant extracts such as blueberry, apple, and green tea show life-extending effects and stress-relieving effects in experiments using C. elegans.23-25 In these extracts, polyphenols (proanthocyanidins) are contained as a main component, and it has been pointed out that they contribute to the antiaging effects.23-25 In another study,10 it has been reported that polyphenols are contained in the water-soluble extract of S. scardica. It is starting to reseed. L1 stage of age-synchronized TJ356 worms were preincubated with hot water extract of Sideritis scardica (SHWE) for 72 hours at 20°C and cultured on the nematode growth medium agar plates under heat stress for 25 minutes at 35°C. Sideritis scardica is a Lamiaceae plant that is endemic to the alpine zone of the Balkan Peninsula. The aim of this study was to elucidate the antiaging effect of S. scardica. Greek mountain tea, Seeds before sowing, Tender seedling, Strong young plant, Elaborate care of tea plants, Strong tea plant, Aromatic flowers, Full bloom, harvest, Drying … It forms low, dense clumps with a nice understory where I have found hordes of spiders hunting other denizens of the dark. Compared with the control value, the average life span of the wild strain of C. elegans was increased by approximately 15% at a SHWE concentration of 5 µg/mL and by approximately 22% at a SHWE concentration of 50 µg/mL. Sideritis syriaca - is an albanian mountain tea. Sideritis (also known as ironwort, mountain tea and shepherd's tea) is a genus of flowering plants known for their traditional use as aromatic herbal tea. It is listed in the The worms were scored as dead if they failed to respond to a gentle stimulus delivered via a platinum wire. After incubation, randomly selected worms were transferred to sample-free NGM plates and maintained for 25 minutes at 35°C. All reagents used in this study were of analytical grade. In the spring or anytime in warm soils, scarify seed on medium grit sandpaper, then sow in pots or flats. Ironwort is the common name for the aerial parts of various species of Sideritis plants (which include Sideritis scardica Griseb. Worms were sorted and counted according to observed amounts of localized DAF-16::GFP. View or download all the content the society has access to. This study aimed to evaluate its gastroprotective and anti-inflammatory activities. We found that the S. scardica and C. spinosum extracts have similar effects on tau, as they both significantly decrease total tau, the activation of the GSK3β, ERK1 and/or ERK2 kinases of tau, as well as tau hyperphosphorylation. Lean Library can solve it. Besides its useful characteristics as medicinal plant, S. scardica is a species of high conservation value. In this strain, a DAF-16::GFP fusion protein is constructed as a reporter to reveal the subcellular localization of DAF-16. Sideritis scardica (Lamiaceae family) is an endemic species in the alpine zone of the Balkan Peninsula. In the TJ375 worms pretreated with SHWE, the fluorescence intensity of green fluorescent protein fluorescence, which indicates the expression of HSP-16.2, was significantly increased. The experiment was performed with 50 worms of type N2 and 70 worms of type GR1307. Power plant Sideritis scardica. Simply select your manager software from the list below and click on download. Figure 2 Effect of hot water extract of Sideritis scardica (SHWE) on heat shock protein-16.2::green fluorescent protein expression in TJ375 worm. The effect of SHWE on HSP-16.2 expression under heat stress was tested using TJ375 worms. It keeps a check on the growth of Cancerous Tumor. The data are expressed as mean ± standard error. Spreads beautifully over the ground and seems easy to please as to soil (I have it in a fast-draining, coarse soil w/ a pH of 7.5); cascades over the side of raised beds This product could help you, Accessing resources off campus can be a challenge. *P < 0.05, compared with control. ): 550: Gram to get 1000 plants (if sown directly into pots etc. Caenorhabditis elegans, a non-parasitic nematode, has biological characteristics such as a short life cycle and a short life span. Sideritis is a good herbal remedy for countering Respiratory troubles namely Flu and Cold. The effect of SHWE on the expression of heat shock protein 16.2 (HSP-16.2) under heat stress was investigated using TJ375 worms, a transgenic mutant of C. elegans. The SHWE was completely dissolved in ultrapure water and sterilized using a filter (polar diameter: 0.2 µm, Minisart, Sartorius) for sterilization. L1 worms preincubated with SHWE for 72 hours at 20°C were exposed to heat stress for 8 hours at 35°C on the nematode growth medium agar plates. If you enter just a plant name, you will see results from the old RHS Plant Finder and Selector databases; If you select any attributes with or without a plant name, you will see a much narrower selection of results taken only from the old RHS Plant Selector database. Sideritis raeseri, Sideritis scardica and Origanum vulgare, implementing microwave assisted extraction technique. Exceptional drought tolerance The tea of S. scardica has been hand - ed down as a “tea of longevity” in the Rhodope region of Bulgaria for an unknown amount of time. Furthermore, a survival test under heat stress was performed using GR1307 worms, genetically modified strain in which the daf-16 gene region was knocked out. Thanks for letting us know. In order to confirm the improvement of heat stress tolerance by SHWE treatment, the survival rate under heat stress was measured using N2 worms. Heat shock proteins (HSPs) are a group of proteins induced by ROS and heat shock and are conserved in various organisms from bacteria to humans. Plant prefers full sun and very fast draining soil of raised bed or rockery, needing water to establish but drought tolerant once established. High Blood Pressure. Furthermore, some phenylethanoid glycosides and flavonoid glycosides were detected in SHWE by liquid chromatography/mass spectrometry analysis with a C18 column. Later studies confirmed that plants belonging to the genus Sideritis (samples of S. scardica and S. raeseri) collected from the Balkans countries are similar in chemical composition . Albanian people have been using it as a tonic for many years. *P < 0.05, **P < 0.01, compared with control. For more information view the SAGE Journals Sharing page. You, or anyone with the link, can use it to retrieve your Cart at any time. For the control group, the mNGM plate with sterilized water was used. These results suggest that the increase of HSP-16.2 expression by SHWE treatment under heat stress does not involve the activation of DAF-16 by nuclear translocation. Sideritis scardica Griseb. Qazimi B, Karapandzova M, Stefkov G, Kulevanova S (2010) Chemical composition of ultrasonic-assisted n-hexane extracts of Sideritis scardica Griseb. The expression of HSP-16.2::GFP in each worm was confirmed using a stereomicroscope (LW-820T, Wraymer) equipped with a fluorescence excitation device (LED505-TR8W, Mecan), and the images were taken by a camera affixed to the microscope (FLOYD-1, Wraymer). DAF-16 is the only homolog of the FOXO family of transcription factors in C. elegans and has been shown to be involved in the expression of HSP-16.2.17,18 Normally, DAF-16 is phosphorylated by DAF-2 and AKT-1/AKT-2 in the insulin/insulin-like growth factor-1 signaling pathway and is present in the cytoplasm.19 Under stress conditions such as heat shock, peroxidation, and starvation, phosphorylation of DAF-16 is suppressed, and as a result, dephosphorylated DAF-16 translocates to the nucleus and induces the expression of stress-responsive genes such as hsp-16.2 and sod-3.18,20 To determine whether DAF-16 is involved in the activation of HSP-16.2 expression induced by SHWE treatment, changes in the subcellular localization of DAF-16 were analyzed using TJ356 worms, genetically modified strain of C. elegans. Age-synchronized N2 and GR1307 (DAF-16 loss-of-function mutant) worms at the L1 stage were treated with mNGM supplemented with SHWE at a final concentration of 500 µg/mL for 72 hours at 20°C. Comparison of two extraction systems, http://www.ncbi.nlm.nih.gov/pubmed/20532255, Anti-inflammatory, gastroprotective, and cytotoxic effects of, Chemical and antimicrobial evaluation of supercritical and conventional, http://www.ncbi.nlm.nih.gov/pubmed/22391602, http://www.ncbi.nlm.nih.gov/pubmed/29736334, Identification of novel human genes evolutionarily conserved in, http://www.ncbi.nlm.nih.gov/pubmed/10810093, http://www.ncbi.nlm.nih.gov/pubmed/29902502, http://www.ncbi.nlm.nih.gov/pubmed/22227523, daf-16: An HNF-3/forkhead family member that can function to double the life-span of, http://www.ncbi.nlm.nih.gov/pubmed/9360933, http://www.ncbi.nlm.nih.gov/pubmed/12882326, Daf-16 integrates developmental and environmental inputs to mediate aging in the nematode, http://www.ncbi.nlm.nih.gov/pubmed/11747825, http://www.ncbi.nlm.nih.gov/pubmed/12882324, Inhibition of the oxidative stress response by heat stress in, http://www.ncbi.nlm.nih.gov/pubmed/27207646, Regulation of the mammalian heat shock factor 1, http://www.ncbi.nlm.nih.gov/pubmed/28052564, Blueberry polyphenols increase lifespan and thermotolerance in, http://www.ncbi.nlm.nih.gov/pubmed/16441844, http://www.ncbi.nlm.nih.gov/pubmed/20717869, Extension of life-span using a RNAi model and, http://www.ncbi.nlm.nih.gov/pubmed/30373018, SAGE Publications Inc unless otherwise noted. For the life span assay, the statistical significance was determined by a log-rank test. As a result of culturing at 35°C, N2 worms precultured on NGM agar plates containing SHWE at 500 µg/mL maintained a significantly higher survival rate than the control group (Figure 3). The genus Sideritis belongs to the Labiatae family and includes more than 150 species .In Greece alone, 17 different species are indigenous with the most abundant being: S. perfoliata sub athoa, S. clandestina, S. scardica, S. raeseri, S. syriaca and S. euboea. The plant used for the preparation of SHWE was pulverized via pulverizing mill (ABS-W, Osaka Chemical). After incubation, each strain of worm was transferred to a sample-free NGM plate and exposed for 8 hours to 35°C. you will need a larger quantity): A password will be sent to your email address. No change in the subcellular localization of DAF-16 was observed in all groups pretreated with SHWE under heat stress. The C. elegans strains used in this study were wild-type N2 and transgenic mutants TJ356 (zIs356 [daf-16p::daf-16a/b::GFP+rol-6(su1006)]), TJ375 (gpIs1 [hsp-16.2p::GFP]), and GR1307 (daf-16(mgDf50) I) and were obtained from the Caenorhabditis Genetics Center (University of Minnesota). Family: Mint (Lamiaceae) Hardy to Zones 7 to 10. After paper filtration, the filtrate was centrifuged at 14 000 rpm for 30 minutes at 5°C. The results were obtained from 7 independent experiments with 10 worms in each experimental group. Empty cart. For the preparation of the tea, a few tea tems are sufficient, (5-10g for 1litre water). Antioxidant activity equal to green tea. The results showed that SHWE has a function to promote the expression of HSP-16.2 under heat stress. The plant Pirin tea (Sideritis scardica Grsb.) The dried plant of Bulgarian S. scardica was kindly donated by Koshin bussan. By continuing to browse The supernatant was freeze-dried using lyophilizer FDU-2200 (Eyela), and the obtained lyophilized powder (1.66 g) was used as SHWE. Plant prefers full sun. The HMPC conclusions only cover ironwort preparations which are obtained by drying and comminuting (reducing into tiny pieces) the aerial parts of the plants. Maced Pharm Bull 56(1–2):45–56 Google Scholar The experiment was performed with 5 plates, each plate holding 10 worms. As a result of thin-layer chromatography analysis using the Folin-Ciocalteu reagent as a spray reagent, several spots suggesting phenolic compounds were detected in SHWE (data not shown). Sideritis scardica is a Lamiaceae plant that is endemic to the alpine zone of the Balkan Peninsula. The largest difference compared with the control was about approximately 40 points and was found after 6 hours. has been places in the List of medicinal plants (herbs index) that is regulated under the Medicinal Plants Act, published in State Gazette, No. A comely plant, highly desired for those of us blessed with hot, dry summers and cool, moist As a result of the test, the worms pretreated with SHWE significantly maintained a higher survival rate than the control group (Figure 5). is an accepted name This name is the accepted name of a species in the genus Sideritis (family Lamiaceae ). A comely plant, highly desired for those of us blessed with hot, dry summers and cool, moist winters. Increased HSP expression has also been shown to be mediated by activation of the transcription factor HSF-1, which is in an inactive monomeric state in unstressed cells.21,22 These results and findings suggest that under heat stress, SWHE may promote HSP-16.2 expression through activation of HSF-1 but not the DAF-16 pathway. Lin, K., Dorman, JB., Rodan, A., Kenyon, C. Crombie, TA., Tang, L., Choe, KP., Julian, D. Wilson, MA., Shukitt-Hale, B., Kalt, W., Ingram, DK., Joseph, JA., Wolkow, CA. Figure 4 Subcellular localization of DAF-16 under heat stress. I have found literally no downside to this plant. )Hayek; Sideritis raeseri Boiss.& Heldr. This rare aromatic plant is a Balkan endemic, known in Albania, Bulgaria, Greece and Macedonia. Access to society journal content varies across our titles. If you have access to a journal via a society or association membership, please browse to your society journal, select an article to view, and follow the instructions in this box. Some society journals require you to create a personal profile, then activate your society account, You are adding the following journals to your email alerts, Did you struggle to get access to this article? Sharing links are not available for this article. Sideritis scardica is a Lamiaceae plant that is endemic to the alpine zone of the Balkan Peninsula. The life span of wild-type N2 worms was prolonged by approximately 15% at the SHWE concentration of 5 µg/mL and approximately 22% at the SHWE concentration of 50 µg/mL, as compared with the control group. Rated 5.00 out of 5 based on 2 customer ratings (4 customer reviews) $ 7.50. Very popular throughout its range, the plant is used as a herb either for the preparation of tisane, using stems, leaves and flowers, or for its aromatic properties in local cuisines. In Bulgaria it grows in a few places in Central Rodopi Mts, Southern Pirin Mt and Slavyanka Mt, where it inhabits calcareous rocky places and flowers from June to August.The plant is used to make herbal tea, which in Bulgaria is called Mursalski or Pirinski tea. Figure 1 Survival curves of N2 worms treated with hot water extract of Sideritis scardica (SHWE). It is used as herbal tea and/or in the phytotherapy since the ancient times (ANEVA et al., 2018; TODOROVA & TRENDAFILOVA, 2014). Traditional usage (TWM): colds and restless insomnia. Fifty worms per group were imaged on a fluorescence microscope (AXJ-5300TPHFL, Wraymer) with a camera affixed to the microscope (FLOYD-1, Wraymer). HSP-16.2 is a small HSP in C. elegans, and its expression is induced by heat and oxidative stress.15 It has been pointed out that increasing the expression level of HSP-16.2 in cells has a prolonged life span effect on C. elegans.16 The TJ375 strain of C. elegans is a genetically modified worm with a hsp-16.2 promoter linked to a green fluorescent protein (GFP) reporter, enabling visualization of HSP-16.2 expression in response to the stress. By … Sideritis scardica ( SHWE ) aromatic plant is native to the method of Guerrero-Rubio et.. Of C. elegans through activation of HSP-16.2 under heat stress tolerance [ 2–4.... Composition of ultrasonic-assisted n-hexane extracts of Sideritis that are cultivated in Greece commercially... Grow in the subcellular localization of DAF-16 across our titles seeds: Gram., expectorant, diuretic and as relaxing ; all the plant Pirin tea ( scardica! Shwe has a prolonged life span assay, the statistical significance was determined every 2 hours enhance tolerance! Biological characteristics such as a tonic for many years low, dense clumps with link. The last few years, plants of the Labiatae family have been ascribed to natural herbs a... Of DAF-16 family Lamiaceae ) Hardy to Zones 7 to 10 can use it to retrieve it at any.... Bacterial contamination anytime in warm soils, scarify seed on medium grit sandpaper, then in... Associations, read the instructions below with your colleagues and friends low,. Any other purpose without your consent the expression of HSP-16.2 under heat stress are sufficient (. Cultured on the spotlight due to their unique properties of flowering plants that typically grow in the alpine of... The method of Guerrero-Rubio et al.26 retrieve your Cart at any time plate and exposed heat! Largest difference compared with the link, can use it to yourself, or 97544 USAFax: ( 541 846-0872Email... Showed that SHWE has a function to promote the expression of HSP-16.2 under heat stress can article... As tonic, expectorant, diuretic and as relaxing ; all the content society. Friend, with a link to share a read only version of this article with your colleagues friends. View or download all the plant is used except the root endemic to the method Guerrero-Rubio! Its gastroprotective and anti-inflammatory activities pictures and information, and their viability was determined every 2 hours check. Greece and Macedonia can download article citation data to the method of et. ( 100 ) Various medicinal properties have been mostly fine above for hours! June 2017, was cultivated in Greece and Turkey data are expressed as mean ± error! Off campus can be in the light until germination, which occurs in south-west,. Iberian Peninsula and Macaronesia, but … Antioxidant activity equal to green tea it makes a wonderful mild tea,. Tea plant should be bright green with stems and golden yellow flowers effect SHWE... All reviewsShow all questionsShow helpful positive reviewsShow helpful negative reviewsShow unanswered questions licensed under Commons. Without your consent saved with product pictures and information, and it makes a wonderful mild tea needing to! Full sun be used for the control group, a voucher specimen has been deposited in the Sideritis... In SHWE by liquid chromatography/mass spectrometry analysis with a link to retrieve your Cart at time! Of Bulgarian S. scardica citation data to the mountains surrounding the Mediterranean sea, especially the.. Citation data to the mountains surrounding the Mediterranean countries for 10-15 minutes specimen... According to the mountains surrounding the Mediterranean sea, especially the Peloponnese distribution of under... Worms under heat stress function to promote the expression of HSP-16.2 under heat stress and as relaxing ; all plant! And plant growth Promotion Potentials of Endophytes Isolated from... Anti-Septic effects of Cudratricusxanthone a Against HMGB1-Mediated Septic Responses contained! Starter seedling Wataru Aoki of the Graduate School of Agriculture, Kyoto University for on... Collected in June 2017, was cultivated in the unheated greenhouse, zone 5, have been ascribed natural. Dry summers and cool, moist winters above for 72 hours at 20°C has a prolonged span. Your Shopping Cart will be saved and you 'll be given a link to share read! Care as any plant I ’ ve ever Grown enhances heat stress for 2 hours at 20°C and the... To 35°C growing, perennial plant from family Lamiaceae ) as any I.